a Record control number WOS:000331696100015

a ISSN 2047-2919

a Original cataloging agency NEU GrandLibrary

a Language code of text/sound track or separate title eng

a Classification number WJ750

b Item number .D96 2014

a Personal name Sahin, Z.

a Title Dynamic Hedgehog signalling pathway activity in germline stem cells

h Medium Electronic Document

c Statement of responsibility, etc Z.Sahin, A.Szczepny, E. A. McLaughlin, M. L. Meistrich, W.Zhou, I.Ustunel, K.L.Loveland.

b Name of publisher, distributor, etc Wıley Blackwell, ,

c Date of publication, distribution, etc 2014.

a Summary, etc Although the contribution of Hedgehog (Hh) signalling to stem cell development and oncogenesis is well recognised, its importance for spermatogonial stem cells (SSCs) has not been established. Here we interrogate adult rat SSCs using an established model in which only undifferentiated spermatogonial cells remain in the testis at 15weeks following irradiation, and spermatogonial differentiation is induced within 4weeks by gonadotrophin-releasing hormone antagonist (GnRH-ant) administration. Synthesis of Hh pathway components in untreated adult rat testes was compared with that in irradiated testes prior to and after GnRH-ant exposure using in situ hybridization. In adult testes with complete spermatogenesis, the Desert Hedgehog ligand transcript, Dhh, was detected in Sertoli cells, some spermatogonia and in spermatocytes by in situ hybridization. Spermatogenic cells were identified as sites of Hh signalling through detection of transcripts encoding the Hh receptor, Ptc2 transcripts and proteins for the key downstream target of Hh signalling, Gli1 and the Hh transcriptional activator, Gli2. Remarkably, the undifferentiated spermatogonia present in irradiated adult rat testes contained Dhh in addition to Ptc2, Gli1 and Gli2, revealing the potential for an autocrine Hh signalling loop to sustain undifferentiated spermatogonial cells. These transcripts became undetectable by in situ hybridization following GnRH-ant induction of spermatogonial differentiation, however, detection of Gli1 protein in spermatogonia in all groups indicates that Hh signalling is sustained. This is the first evidence of active Hh signalling in mammalian male germline stem cells, as has been documented for some cancer stem cells.

a Topical term or geographic name as entry element Prostate

a Topical term or geographic name as entry element Near East University Article

a Topical term or geographic name as entry element Yakın Doğu Üniversitesi Makale

g Relationship information Mar 2014, Vol.2, Issue:2, p. 267-274.

t Title Andrology

x International Standard Serial Number 20472919

u Uniform Resource Identifier http://library.neu.edu.tr:2048/login?url=http://dx.doi.org/10.1111/j.2047-2927.2014.00187.x